Fig. 5.

Sleep behavior of Rpt2 knockdown flies. Actograms are shown on top of each column; quantization of the corresponding actograms and periods are shown in the lower panels. Panels A, B, and C show overall accumulated sleep-time during light and dark periods for days 3 to 5, 8 to 10, and 15 to 17 respectively. Sleep-time in RNAi-Rpt2 (experimental) flies (red) is reduced only after the fourth day of aging. In every 3 day period shown, RNAi-Rpt2 animals (red) accumulated significantly less sleep-time than controls (black). D) Comparison of accumulated sleep-time during the light and dark periods for days 3 and 4, and day 5 (black control, red RNAi-Rpt2 experimental) show that at day 5, RNAi-Rpt2 flies have significantly reduced sleep-time accumulation compared to controls. Panels E (light period) and F (dark period) show accumulated sleep-time for days 3 to 5, a significant sleep-time decrease in RNAi-Rpt2 flies (red) can only be observed after the fourth day of aging and only in the light period. G) Accumulated sleep-time for days 8 to 10 quantified during the light and dark period. No significant differences were found in this period in accumulated sleep-time between RNAi-Rpt2 and control. Panels H (light period) and I (dark period) show accumulated sleep-time for days 8 to 10. A significant decrease in sleep-time accumulation was observed only during the light period in RNAi-Rpt2 animals. J) Accumulated sleep-time for days 15 to 17 quantified during the light and dark period showed a significant decrease in sleep-time in RNAi-Rpt2 flies. Panels K (light period) and L (dark period) show accumulated sleep-time for days 15 to 17. In both periods RNAi-Rpt2 flies had a significant decrease in sleep-time accumulation. Significance was tested for each independent period using unpaired t-test, n ≥23, Bar graph = mean with SEM (* = P < 0.05, ** = P < 0.01, *** = P < 0.001, **** = P < 0.0001).