Skip to main content
. 2020 Jul 11;9:65–77. doi: 10.1016/j.ibror.2020.07.001

Fig. 6.

Fig. 6

Neuronal degeneration induced by the Rpt2 knockdown in dopaminergic neurons. Panels A (2 days after eclosion) and B (16 days after eclosion) show representative anti-TH immunofluorescence maximum intensity z-projections of confocal images of control adult Drosophila brains. Panels C (2 days after eclosion) and D (16 days after eclosion) show representative anti-TH immunofluorescence maximum intensity z-projections of confocal images of experimental (Rpt2 knockdown) Drosophila brains. Panels E and F show quantization of the different dopaminergic neuron clusters revealed by anti-TH immunofluorescence. Dopaminergic neuron degeneration can be observed in all clusters but PAL and PPM1/2. Dopaminergic neurons were quantified from each hemisphere independently. n = 12 hemispheres, * = P < 0.05, ** = P < 0.01, *** = P < 0.001, **** = P < 0.0001, Bar graph = mean with SEM. Significance was tested using unpaired t-test. Inlay between confocal images is a map of the stereotypical dopaminergic neuron clusters (modified from Kasture et al., 2018).