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. 2020 Jul 12;12:5651–5658. doi: 10.2147/CMAR.S258887

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© 2020 Hao et al.

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Circ-ITCH serves as a ceRNA for miR-421 in OSCC. (A) The subcellular distribution of circ-ITCH in OSCC cells. (B) The predicted binding site of miR-421 within circ-ITCH fragment. (C) Dual-luciferase reporter assay for the luciferase activity in HEK-293 cells. (D) RT-qPCR analysis for the expression levels of miR-421 in OSCC cells after transfection. (E) RT-qPCR analysis for the expression levels of miR-421 in OSCC tissues and adjacent normal tissues. (F) RT-qPCR analysis for the expression levels of miR-421 in OSCC cell lines and normal HOK cells. (G) Pearson correlation analysis for the correlation between the expression levels of circ-ITCH and miR-421 in OSCC tissues. *P<0.05 vs. miR-NC-transfected cells; ^#P<0.05 vs. empty vector-transfected cells; ^&P<0.05 vs. normal tissues or HOK cells.