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. 2020 Aug 1;342:108824. doi: 10.1016/j.jneumeth.2020.108824
Syringe Preparation:
Borosilicate glass capillary WPI, 1B100−3
Pipette puller Narishige, PC-10
Microforge Narishige, MF-830
Mineral oil Sigma Aldrich, 330,779
Hamilton syringe, 5 μL Model 75, 7634−01 Hamilton Company
Dual Ferrule Adaptors Hamilton, 55751−01
1 mL Syringe and 30G Needle Becton Dickinson; 303172, 304000
Parafilm(R)M Bemis, PM-996
Surgery Cover Buster, 141765
Surgical setup:
Small Animal Stereotaxic Instrument Kopf, Model 940
Microinjection Unit Kopf, Model 5000
Coarse Adjustment Handle Kopf, Model 5000-G
Anaesthetisation Mould Custom
Illumination System Schott KL1500 LCD
Medical Wipes Kimberley Clark 7432
Absorbent Pad Vettech, FM041
3M Transpore Surgical Tape
Permanent Marker
Anaesthesia:
Induction chamber Vettech, AN010S
Isoflurane setup Vettech Isoflurane Key Fill Vapouriser 5%
Isoflurane Forane
Isoflurane Key Applicator Vettech AN003B
Oxygen Concentrator Vettech AN037
Aldasorber Scavenger Vettech AN006A & Active Scavenging Unit