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. 2020 Jul 14;53(1):217–232.e5. doi: 10.1016/j.immuni.2020.06.013
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Goat F(ab’)2 Anti-Human Kappa-UNLB Southern Biotech Cat#2062-01; RRID: AB_2795736
All antibodies used for cytometry Various Table S1

Biological Samples

Leukocyte reduction chamber from human blood Stanford Blood Center stanfordbloodcenter.org
Whole blood from healthy donors Obtained under informed consent with IRB approval n/a
Human tonsil Stanford Hospital, adult tonsilectomy from adults with obstructive sleep apnea stanfordhealthcare.org
Human lymph node Stanford Hosptial, biopsy sample of recovered lymphoma patient stanfordhealthcare.org
Human bone marrow from healthy donors AllCells allcells.com

Chemicals, Peptides, and Recombinant Proteins

Ficoll-Paque Plus GE Healthcare Cat#300-25
RPMI 1640 media Thermo Fisher Scientific Cat#21-870-092
GlutaMAX supplement Thermo Fisher Scientific Cat#35050-061
Fetal bovine serum USDA approved lot Omega Scientific, Inc. Cat#FB-01
Benzonase nuclease Sigma-Aldrich Cat#E1014-25KU
Recombinant human CD40L BioLegend Cat#591704
Human TruStain FcX (Fc Receptor Blocking Solution) BioLegend Cat#422302
Bovine Serum Album (BSA) Heat-shock Treated Thermo Fisher Scientific Cat#BP1600-100
Cell-ID Intercalator-Ir Fluidigm Cat#201192A
Cell-ID Cisplatin Fluidigm Cat#201064
Saponin from quillaja bark Sigma-Aldrich Cat#S7900-25G
Calibration Beads, EQ, Four Element Fluidigm Cat#201078
7-AAD Viability Staining Solution BioLegend Cat#420404
Streptavidin Particles Plus BD Biosciences Cat#557812

Critical Commercial Assays

Cell-ID 20-Plex Pd Barcoding Kit Fluidigm Cat#201060
Maxpar X8 Antibody Labeling Kit Fluidigm Cat#201176B
IMag Cell Separation Magnet BD Biosciences Cat#552311
QIAamp DNA Micro Kit QIAGEN Cat#56304
immunoSeq Human B Cell - survey resolution Adaptive adaptivebiotech.com

Deposited Data

Mass cytometry data This study flowrepository.org/id/FR-FCM-Z2MA
flowrepository.org/id/FR-FCM-Z2MC

Software and Algorithms

RStudio Rstudio rstudio.com
MATLAB MathWorks mathworks.com/products/MATLAB.html
Cytobank Cytobank cytobank.org
CellEngine Primity Bio cellengine.com

Other

Custom code for analysis This study github.com/davidrglass/atlas