REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Cy3-conjugated anti-Pfs25 antibody	(Ruecker et al., 2014)	N/A
Mouse anti-HA antibody	Sigma-Aldrich	Cat# H3663; RRID: AB_262051
Rabbit anti-GAPDH antibody	Abcam	Cat# ab9485; RRID: AB_307275
Anti-mouse antibody	Thermo Fisher Scientific	Cat# 62-6520; RRID: AB_2533947
Anti-rabbit antibody	Cell Signaling Technology	Cat# 7074S; RRID: AB_2099233
Bacterial and Virus Strains
Escherichia coli Rosetta2-pLysS cells	EMD Millipore	https://www.emdmillipore.com/
Biological Samples
HEK-293 human embryonic kidney cell line	ATCC	https://www.atcc.org
K-562 human bone marrow cell line	ATCC	https://www.atcc.org
Hep G2 human hepatocellular carcinoma cell line	ATCC	https://www.atcc.org
Placenta (tissue protein extract)	ATCC	https://www.atcc.org
Chemicals, Peptides, and Recombinant Proteins
All tested antimalarials and their structures are available in Figure S1.		N/A
MMV030084 (TCMDC-140369)	Medicines for Malaria Venture, Geneva, Switzerland	N/A
TCMDC-140680	GlaxoSmithKline, Tres Cantos, Spain	N/A
TCMDC-141334	GlaxoSmithKline, Tres Cantos, Spain	N/A
TCMDC-141060	GlaxoSmithKline, Tres Cantos, Spain	N/A
TCMDC-141070	GlaxoSmithKline, Tres Cantos, Spain	N/A
TCMDC-140700	GlaxoSmithKline, Tres Cantos, Spain	N/A
TCMDC-140762	GlaxoSmithKline, Tres Cantos, Spain	N/A
TCMDC-140549	GlaxoSmithKline, Tres Cantos, Spain	N/A
TCMDC-141154	GlaxoSmithKline, Tres Cantos, Spain	N/A
MMV030734 (TCMDC-141019)	Medicines for Malaria Venture, Geneva, Switzerland	N/A
BrightGlo reagent	Promega	https://www.promega.com/
MitoTracker Deep Red	Thermo Scientific	https://www.thermofisher.com/
D-Luciferin	PerkinElmer	https://www.perkinelmer.com/
TMTsixplex reagents	Thermo Scientific	https://www.thermofisher.com/
AKTA purifier	GE Healthcare	https://www.gehealthcare.com/
NHS-activated Sepharose matrix	Sigma-Aldrich	https://www.sigmaaldrich.com/
Mini-PROTEAN® TGX™ Precast Gels	Bio-Rad	https://www.bio-rad.com/
SuperSignal® West Pico Chemiluminescent substrate	Thermo Fisher Scientific	Cat# PI34080
anhydrotetracycline	Sigma-Aldrich	Cat# 37919
ML10	LifeArc	https://www.lifearc.org/
WR99210	Sigma-Aldrich	https://www.sigmaaldrich.com/
Critical Commercial Assays
ADP-Glo Kinase Kit	Promega	https://www.promega.com/
Nextera XT kit	Illumina	https://www.illumina.com/
Peptide substrate GRTGRRNSI-NH2	Sigma-Aldrich	Cat# SCP0212
Renilla-Glo(R) Luciferase Assay System	Promega	Cat# E2750
QiAmp DNA Blood Mini kit	Qiagen	https://www.qiagen.com/
Deposited Data
PKG crystal structure	(Baker et al., 2017)	PDB ID: 5DYK
Experimental Models: Organisms/Strains
Anopheles stephensi mosquitoes	Insectary Core Facility, New York University	N/A
Plasmodium falciparum asexual blood stage parasites	Goldberg lab, Washington University	3D7-A10 clone
P. falciparum asexual blood stage parasites	Fidock lab, Columbia University Medical Center	Dd2-B2 clone
P. falciparum NF54 CDPK1 T145M line	Miller lab, Malaria Cell Biology, NIAID, NIH (Bansal et al., 2018)	CDPK1T145M
P. falciparum 3D7elo1-pfs16-CBG99 transgenic parasites	Fidock lab, Columbia University Medical Center (Cevenini et al., 2014)	N/A
BALB/c female mice	The Jackson Laboratory	https://www.jax.org/
P. berghei ANKA (Pb-Luc)	Insectary Core Facility, New York University	Pb-Luc, also known as P. berghei-ANKA-GFP-Luc-SMCON
Oligonucleotides
All primers and cloning fragments are shown in Table S1 (J) and (K)		N/A
Recombinant DNA
pSN054 vector	(Nasamu et al., 2019)	N/A
pET28-PfTKL3-SAM(V54E)-KD	(Abdi et al., 2010)	N/A
Software and Algorithms
GraphPad Prism Version 8	GraphPad Software, San Diego, CA, USA	www.graphpad.com
ICY BioImage Analysis	(Delves et al., 2016)	http://icy.bioimageanalysis.org/
R Studio	RStudio Team, 2015	http://www.rstudio.com/
Proteome Discoverer	Thermo Scientific	https://www.thermofisher.com/us/en/home.html
Mascot	Matrix Science	http://www.matrixscience.com/
Scaffold Q+S	Proteome Software	http://www.proteomesoftware.com/
ChemiDoc™ MP System and Image Lab 5.2.0	Bio-Rad	https://www.bio-rad.com/
El- MAVEN software	(Agrawal et al., 2019)	https://elucidatainc.github.io/ElMaven/
Schrodinger molecular modeling suite	Schrodinger	http://www.schrodinger.com/