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PPT treatment enhanced the multi-caspase activity in ESCC cells. The cells were treated with the indicated concentrations of PPT (0.2, 0.3, and 0.4 µM) or DMSO. The activation of multi-caspases was measured using a Muse™ Multi-Caspase Kit (Merck Millipore) and a Muse™ Cell Analyzer (Merck Millipore). The multi-caspase assays were conducted in triplicate.
