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. 2020 Jun 27;21(13):4575. doi: 10.3390/ijms21134575

Figure 5.

Figure 5

Collagen IV expression following cardiotoxin (CTX)-induced muscle damage in young and aged skeletal muscle. (A) Immunostaining of collagen IV (green) and DAPI (blue) at each time point following damage. (B) Collagen IV positive area in the undamaged contralateral leg was not significantly difference in young and aged muscle (t-test: p = 0.83). No significant differences in collagen IV percent positive area were detected in (C) necrotic and (D) regenerating regions. (E) Magnified of collagen IV area in young and aged muscle. Arrows denote regions of thicker collagen IV layers at five days post-CTX in aged muscle. (F) A significant interaction between age and recovery time point was observed (p = 0.015). Simple main effects post-hoc analysis found aged muscle to have a significantly greater collagen IV layer thickness compared to young at five and seven days following damage (p < 0.001 in both instances). * indicates significant differences detected by a simple main effects post-hoc analysis (p < 0.001). n = 4–5 per group. Data presented are means ± standard deviation. Scale bar represents 50 μm.