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. 2020 Jul 1;21(13):4701. doi: 10.3390/ijms21134701

Figure 6.

Figure 6

The ChIP result of 35S-GmbZIP19–GFP transient expressing soybean. (A) qRT-PCR analysis of biotic stress-related genes in 35S-GmbZIP19–GFP transient expressing soybean. (B) qRT-PCR analysis of abiotic stress-related genes in 35S-GmbZIP19–GFP transient expressing soybean. (C) ChIP-qPCR analysis of GmbZIP19 binding to abiotic stress-related genes using GFP antibody and 35S-GmbZIP19–GFP transient expressing soybean. (D) ChIP-qPCR analysis of GmbZIP19 binding to biotic stress-related genes using GFP antibody and 35S-GmbZIP19–GFP transient expressing soybean. Three independent biological replicates were performed. The error bars indicate ± SD (n = 3 replicates). Asterisks indicate significant differences for the indicated comparisons based on a Student’s t-test (** p < 0.01; * p < 0.05). (E) The yeast one-hybrid result of GmbZIP19. Three independent biological replicates were performed.