Table 5.

Assay validation parameters Limit of Blank (LoB), Limit of Quantification (LoQ), and Limit of Detection (LoD).

Gene	LoB	LoQ	LoD
CDH1	0.55 RFU	2.89 RFU	69.0 pg/µL
CDKN2Ap16	0.50 RFU	2.78 RFU	11 pg/µL
RASSF1A	0.81 RFU	3.38 RFU	54.75 pg/µL
TERT	1.00 RFU	3.96 RFU	0.94 pg/µL
WT1	0.56 RFU	2.57 RFU	1.45 pg/µL