Figure 2.

Fluorescence analysis to detect editing of the PTC (UGA) in HeLa cells expressing the H2BGFP^opal gene. HeLa-H2BGFP^opal cells were co-transfected with dCAS13b/ADAR2_DD and selected gRNAs coding plasmids (450 ng total DNA). Two days after transfection, cells were fixed with methanol for 5 min and the green fluorescence due to H2BGFP expression was monitored using the 63X objective on a ZEISS microscope equipped for epifluorescence. Nuclei were visualized with DAPI (blue). HeLa H2BGFP^WT cells and untransfected H2BGFP^opal cells were used as positive and negative control, respectively.