Figure 7.

Proliferation of ADSCs on the surface of the NPs-loaded scaffolds upon nitrogen plasma exposure (16 s in HBSS; cells left in HBSS for 3 h after plasma treatment): (a) cell number increase with time determined by nuclei counting (control—biomaterial without any NPs; 0.25—concentration (wt.%) of NPs within the structure of the biomaterial; * statistically significant results compared to the corresponding (with or without plasma activation) control biomaterial, # compared to the corresponding (with or without plasma activation) biomaterial containing Fe_xO_y/MSNPs; $ statistically significant results between plasma-treated and untreated biomaterials, according to One-way Anova followed by Tukey’s test, p < 0.05); (b) CLSM images of ADSCs on the surface of the biomaterials (cells at extremely low concentration were seeded) 6 days after plasma treatment upon fluorescent staining of cytoskeleton and nuclei (red fluorescence—cytoskeleton, blue fluorescence—nuclei, magn. 40×).