Skip to main content
. 2020 Jun 30;17(13):4716. doi: 10.3390/ijerph17134716

Figure 5.

Figure 5

GR extracts attenuated the LPS-induced inflammatory responses in 3T3-L1 adipocytes and adipocytes exposed to macrophage-conditioned media (CM). The 3T3-L1 cells were seeded and induced to differentiation in the presence of dimethyl sulfoxide (DMSO, vehicle control) and GRE (100 μg/mL) for 7 days and then starved in DMEM for 12–18 h, followed by LPS stimulation (100 ng/mL, 2h) in the presence or absence of GRE. (A) IL-6, IL-1β, and TNFα gene expression by qPCR. (B) Protein extracts from LPS-treated 3T3-L1 adipocytes in the presence or absence of GRE (100 μg/mL) were immunoblotted with antibodies targeting NF-kB, p-JNK, p-ERK, p-p38, t-ERK, or β-actin (loading control); 3T3-L1 adipocytes were preincubated with DMSO or GRE before receiving the CM of LPS-treated BMDMs with or without GRE. (C) Experimental scheme. (D) IL-6, MCP1, and TNFα gene expression by qPCR. All values are presented as the mean ± SEM (n = 3–4/group for each experiment). #### p < 0.0001; compared with non-macrophage (mφ) CM vs. mφ CM. * p < 0.05; *** p < 0.001; **** p < 0.0001 compared with LPS-treated group or m mφ CM vs. GRE-treated group by one-way ANOVA with Bonferroni’s comparison test. +, treatment; −, non-treatment.