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. 2020 Jun 27;21(13):4583. doi: 10.3390/ijms21134583

Figure 2.

Figure 2

GC7 has an inhibitory effect on p53 expression. (A) The HCT-116 cells were treated with GC7 at a concentration of 80 μM for 48 h and subject at UV-C irradiation where indicated. Cell lysates were analyzed by immunoblotting with the antibodies showed in the figure. (B) The p53 expression level was normalized to GAPDH by the Bio-rad Image Lab Software 5.2.1 and the value of p53 in untreated cells was set as 1. Data are means ± SD (n = 5). p values reported to the upper side of the histograms were calculated versus control group. (C) Treatment of HCT-116 cells with GC7 at a concentration of 80 μM 48 h inhibited p53 expression induced by doxorubicin 1 μM. Whole cell extracts were prepared and analyzed by immunoblotting with the indicated antibodies. Intensity of bands was detected by the Bio-rad Image Lab Software 5.2.1 and the signal of interest was normalized to control GAPDH. The value of p53 in untreated cells was set as 1. Data are means ± SD (n = 4). p values reported to the upper side of the histograms were calculated versus control group. (D) Treatment of MCF-7 breast cancer cell lines with GC7 (80 μM) for 48 h and UV-C irradiation at the indicated dose. Whole cell extracts were prepared and analyzed by immunoblotting with the indicated antibodies. Each image is representative of at least three independent experiments.