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Human skin equivalent generated using PCS-200-010, PCS-200-013, and PCS-201-010 cell lines and differentiated for 14 days. Formalin-fixed, paraffin-embedded 3-dimensional (3D) skin models. Fluorescent immunostaining. Green—Cytokeratin 14 (Abcam ab77684). Melanocytes are seen as the dark color of the entire surface of the epidermis (green) layer. (A) brightfield, (B) Cytokeratin 14 (green), (C) merge.
