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. 2020 Jun 28;21(13):4592. doi: 10.3390/ijms21134592

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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DNA map of O157:H7 PA20 pchE and promoter sequences showing binding sites for relevant primers used in this study. Positions and designations of pchE and upstream genes are annotated using the E. coli O157:H7 Sakai reference strain (accession #NC_002695.1). Numbers 1 and 2 designate gene-specific primers, pchE_GSP1 and pchE_GSP2, used for mapping the PA20 pchE transcriptional start site (tss). Numbers 3 and 4 designate primers 15881034F and 15881034R, respectively, used to amplify the DNA region 5′ to pchE for incorporation with lacZ in plasmid pMLB1034::pchE.