Figure 6.

Osteoinductive and osteoconductive activities of the GO-P hybrid scaffold in vitro. (A) AdGFP or AdBMP9-infected iMADs were mixed with PPCNg (a) or GO-P (b) and examined at 48 h after infection under bright field (BF) or GFP fluorescence microscope (GFP). Representative images are shown. (B,C) ALP activity analysis. AdGFP or AdBMP9-transduced iMADs were mixed with PPCNg or GO-P and seeded in 24-well plates. ALP staining was carried out on day 5 (B), while quantitative ALP assay was conducted at 3, 5, and 7 days after infection (C). All assays were done in triplicate. * p < 0.05 and ** p < 0.01 when compared to respective GFP groups. Reproduced from [73]. Copyright © 2018 American Chemical Society.