Figure 3.

Effects of PH on the protein expression levels of TRP-1, TRP-2, and tyrosinase in B16F10 cells. Cells were treated with the indicated concentrations of PH for 24 h. α-MSH (200 nM) and arbutin (100 μM) were used as a negative and positive control, respectively. Protein levels were examined by Western blotting. (a) Western blotting and protein levels of (b) TRP-1, (c) TRP-2, and (d) tyrosinase. Results are expressed as percentages of untreated cells. All experiments were carried out in triplicate, and the results are expressed as the mean ± SD. ^## p < 0.01 in comparison with the untreated cell group and ** p < 0.01 compared with the α-MSH-treated group.