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. 2020 Jul 2;21(13):4734. doi: 10.3390/ijms21134734

Figure 1.

Figure 1

Western blot (WB) analysis of the effect of Dentin Sialophosphoprotein (DSPP) and Matrix Metalloproteinase 20 (MMP20) silencing on MMP2 and MMP9 levels. (a) Downregulation of MMP2 in DSPP, MMP20, and combined DSPP was significant (ShDSPP = p < 0.01; shMMP20 = p < 0.001; shDM = p < 0.05) when compared to the control. (b) Similarly, downregulation of MMP9 in DSPP, MMP20, and combined DSPP–MMP20 was significant (shDSPP = p < 0.01; shMMP20 = p < 0.001; shDM = p < 0.05) when compared to the control. Beta actin was used as the internal control. Values are given as mean ± SE for 3 independent experiments. shC = Scrambled Control; shDSPP = DSPP Silenced OSC2 cells; shMMP20 = MMP20 Silenced OSC2 cells; DM = Combined DSPP–MMP20 Silenced OSC2 cells; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.