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. 2020 Jul 2;21(13):4734. doi: 10.3390/ijms21134734

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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WB analysis of kallikreins levels in DSPP and MMP20 silenced OSC2 cells. (a) Downregulation of KLK4 in shDSPP (60%), shMMP20 (40%), and shDM (43%) were significant (p < 0.001, 0.01) compared to the control. (b) Downregulation of KLK5 in shDSPP (66%), shMMP20 (73%), and shDM (35%) were significant (p < 0.05) compared to the control. (c) Downregulation of KLK8 in shDSPP (53%), shMMP20 (35%), and shDM (47%) were significant (p < 0.001, 0.05, 0.01) when compared to the control. (d) Downregulation of KLK10 in shDSPP (48%), shMMP20 (37%), and shDM (73%) were significant (p < 0.05, 0.01) when compared with the control. Beta actin was used as the internal control. Values are given as mean ± SE for 3 independent experiments. shC = Scrambled Control; shDSPP = DSPP Silenced OSC2 cells; shMMP20 = MMP20 Silenced OSC2 cells; DM = Combined DSPP–MMP20 Silenced OSC2 cells; *p < 0.05; ** p < 0.01; *** p < 0.001.