Figure 3.

Aspirin evokes rapid and persistent depolarization in a ROS-dependent manner. (A‒D) (A,B) A375 and (C,D) A2058 cells loaded with DiBAC4(3) were washed, resuspended in HBSS, and treated with the indicated concentrations of aspirin (ASA) or TRAIL (100 ng/mL). (E) A375 cells loaded with the same probe were treated with the indicated concentrations of ASA in the absence or presence of NAC and catalase. The cells were measured for their fluorescence for 3 min in a microplate fluorescence reader with excitation and emission at 485 and 538 nm, respectively. The trace with the vehicle alone is considered as a baseline. Data are shown as F/F₀, where F and F₀ are the sample’s fluorescence at each time point and time zero, respectively. Graphs in (B,D,E) represent the mean ± SD of the average F/F₀ (n = 3). ### p < 0.001; n.s., not significant vs. baseline. * p < 0.05; *** p < 0.001.