Figure 5.

Insulin resistance does not impair muscle glycolytic flux, or overload-stimulated muscle lactate secretion. After 12 weeks of high-fat diet (HFD) feeding, mice underwent unilateral synergist ablation surgery to induce overload (OVL) of the plantaris muscle for 5 days. (A) Muscles were incubated in [5-³H]-glucose to determine glucose flux through glycolysis. (B) Buffer lactate levels were measured at the end of the glycolytic flux assay as an indicator of muscle lactate secretion. Data are mean ± standard deviation. Statistical analysis was performed using a 2-way ANOVA with Tukey’s post-hoc analysis. N = 12 muscles/group. p < 0.05 # vs. Sham (Sh).