REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
anti-CD11c-FITC (clone HL3)	BD PharMingen	Cat#553801; RRID:AB_395060
anti-CD19-eFluor®450 (clone 1D3)	eBioscience	Cat#48-0193-82; RRID:AB_2734905
anti-CD25-FITC (clone 7D4)	BD PharMingen	Cat#553072; RRID:AB_394604
anti-CD25-PerCPCy5.5 (clone PC61)	BD PharMingen	Cat#551071; RRID:AB_394031
anti-CD3-eFluor®450 (clone 17A2)	eBioscience	Cat#48-0032-80; RRID:AB_1272229
anti-CD4-APC (clone RM4-5)	BD PharMingen	Cat#553051; RRID:AB_398528
anti-CD4-PE-Cy7 (clone RM4-5)	BD PharMingen	Cat#552775; RRID:AB_394461
anti-CD69-eFluor®450 (clone H1.2F3)	eBioscience	Cat#48-0691-82; RRID:AB_10719430
anti-CD69-PE (clone H1.2F3)	BD PharMingen	Cat#553237; RRID:AB_394726
anti-CD86-PE (clone GL1)	BD PharMingen	Cat#553692; RRID:AB_394994
anti-CD8a-PerCP-Cy5.5 (clone 53-6.7)	eBioscience	Cat#45-0081-82; RRID:AB_1107004
anti-CD8α-Pacific Blue (clone 53-6.7)	BD PharMingen	Cat#558106; RRID:AB_397029
anti-CD8α-PeCy7 (clone 53-6.7)	BD PharMingen	Cat#552877; RRID:AB_394506
anti-MHC I (H-2Kb)-FITC (clone AF6-88.5.5.3)	eBioscience	Cat#11-5958-80; RRID:AB_11151335
anti-MHC II eFluor®450 (clone AF120.1, eBioscience)	eBioscience	Cat#48-5320-80; RRID:AB_10671538
anti-vβ 5.1 5.2 TCR-PE (clone MR9-4)	BD PharMingen	Cat#553190; RRID:AB_394698
anti-Vα2 TCR-eFluor®450 (clone B20.1)	eBioscience	Cat#48-5812-82; RRID:AB_10804752
anti-Vα2 TCR-PeCy7 (clone B20.1)	BD PharMingen	Cat#560624; RRID:AB_1727584
Chemicals, Peptides, and Recombinant Proteins
β-lactamase	Sigma-Aldrich	Cat#P0389
BODIPY	ThermoFisher	Cat#D-3922
Chloroquine	Sigma-Aldrich	Cat#C6628
DbeQ	Sigma-Aldrich	Cat#SML0031
Dextran, 3000 MW Tetramethylrhodamine-labeled	ThermoFisher	Cat#D3307
E64	Sigma-Aldrich	Cat#E3132
Eeyarestatin I	Sigma-Aldrich	Cat#E1286
Importazole	Sigma-Aldrich	Cat#SML0341
NMS-873	Selleckcheck	Cat#S7285
Ovalbumin (endotoxin-free)	Hyglos	Cat#300036
Ovalbumin (endotoxin-free)	Invivogen	Cat#vac-pova
Ovalbumin (grade VII)	Sigma Aldrich	Cat#A7641
PR-619	Sigma-Aldrich	Cat#SML0430
Prazosin (in vitro experiments)	Sigma-Aldrich	Cat#P7791
Prazosin (in vivo experiments)	Sigma-Aldrich	Cat#1554705
Prazosin-BODIPY	ThermoFisher	Cat#B7433
SCREEN-WELL® FDA approved drug library V2	Enzo	Cat#BML-2843-0100
Tamoxifen	Sigma-Aldrich	Cat#T9262
β-lactamase	Sigma-Aldrich	Cat#P0389
Critical Commercial Assays
CCF2-FA	ThermoFisher	Cat#K1034
Fixable Viability Dye eFluor® 780	eBioscience	Cat#65-0865-14
LiveBLAzer FRET-B/G Loading Kit	ThermoFisher	Cat#K1095
Deposited Data
Proteomics data: whole cell proteomics of MutuDCs, organellar mapping of MutuDCs	This paper	http://dc-biology.mrc-lmb.cam.ac.uk
Experimental Models: Cell Lines
B16-OVA	Falo et al., 1995	RRID:CVCL_WM78
B3Z	Sanderson and Shastri, 1994	RRID:CVCL_6277
MC38-OVA	Gilfillan et al., 2008	N/A
MutuDC	Fuertes Marraco et al., 2012	N/A
Experimental Models: Organisms/Strains
Mouse: C57BL/6J (wild type)	Charles River	Cat#632
Mouse: C57BL/6-Rag1tm1MomTg(TcraTcrb)1100Mjb	Taconic	Cat#4175
Mouse: C57BL/6-Tg(TcraTcrb)1100Mjb/Crl (OT-1)	Charles River	Cat#642
Mouse: C57BL/6-Tg(TcraTcrb)425Cbn/Crl (OT-2)	Charles River	Cat#643
Mouse: NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG™)	Jackson Laboratory	Cat#005557
Software and Algorithms
ImageJ/FIJI	Schindelin et al., 2012	https://imagej.nih.gov/ij/
GraphPad Prism version 8.4.2	GraphPad Software, La Jolla California USA	https://www.graphpad.com
MaxQuant version 1.6	Cox and Mann, 2008	https://www.maxquant.org/
Perseus version 1.6	Tyanova et al., 2016	https://www.maxquant.org/
R version 3.5.3	The R Foundation	https://www.R-project.org/
Tidyverse version 1.3.0	Wickham et al., 2019	https://www.tidyverse.org/