Figure 5.

Social isolation: reduced response in social space in nlg3^Def1 flies, but no effect on protein levels in Canton-S. (A–D) Mean number of flies within 4BL ± s.e.m. in Cs, nlg3^Def1 and nlg3^L04. n = 9 trials with 12–17 flies per trial. (A) Single housed Cs and nlg3^Def1 had a lower number of flies within 4BL after being single housed compared to group housed (two-way ANOVA—effect of isolation: F_1,32 = 24.56, p < 0.0001); however SH nlg3^Def1 did not have as drastic a decrease compared to Cs (two-way ANOVA—interaction between isolation and genotype: F_1,32 = 4.96, p = 0.0331). (B) Similar results as the males were obtained with nlg3^Def1 females (two-way ANOVA—effect of isolation: F_1,28 = 15.10, p = 0.0006; two-way ANOVA—interaction between isolation and genotype: F_1,28 = 5.166, p = 0.0309). (C) Nlg3 protein abundance in Cs males did not change in SH compared to GH flies in either protein isoform. The same Nlg3 expression pattern was observed in females. (D) Representative Western blots for Cs male and female GH or SH. Male treatments are relative to GH Nlg3-FL; males and female treatments are relative to GH Nlg3-FL females. GH: group housed, SH: single housed. All samples are normalized to total protein. n = 4 for all Western.