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Overview of the experimental design. Rapamycin was administered continuously for 12 h or 24 h to the cell cultures; then, it was withdrawn for 24 h up to 14 d. Thus, the first time interval of rapamycin withdrawal (24 h) is indicated as 1 d and it corresponds to rapamycin administration for 24 h followed by 24 h of withdrawal. All later time intervals are reported considering the days the rapamycin withdrawal time lasted.
