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Rapamycin increases the expression of autophagy related genes in U87MG cells. (A) RT-PCR for LC3, and (B) RT-PCR for BECLIN1. * p ≤ 0.05 vs. Control. ** p ≤ 0.05 vs. Control and 10 nM rapamycin. Each RT-PCR was performed in triplicate and confirmed in two independent experiments using both beta-globin and beta-actin as internal references.
