Figure 7.

NLRC4 is occasionally detected in astro- and microglia cells after cerebral ischemia in rats. The co-localization of NLRC4 was evaluated by IF-co-staining in the sham and vehicle group (tMCAO+24 h reperfusion) with astrocytes (GFAP) (a) and microglial cells (Iba1) (b). (a) In astrocytes, low co-localization was evident in sham animals but occasionally appeared in the peri-infarct area in the vehicle group (see 400× magnification and the detail box). (b) For Iba-1 stained microglia, a similar result was observed that after cerebral ischemia, a slight amount of microglia seemed to co-localized with NLRC4 (see arrows in the detail box). Note that for proper visualization, exposure was adapted to show the co-localization between GFAP or Iba-1 positive cells with NLRC4. White bars indicate a scale of 250 µm (200× magnification of sham and vehicle) or 100 µm (400× magnification). The dashed line delineates the transition zone of the peri-infarct area around the ischemic core (asterisk). GFAP–glial fibrillary acidic protein; Iba1–ionized calcium-binding adapter molecule 1.