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. 2020 Feb 20;27(8):2451–2467. doi: 10.1038/s41418-020-0515-2

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© The Author(s), under exclusive licence to ADMC Associazione Differenziamento e Morte Cellulare 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Percent of cells with high-scatter parameters (high SC), in Cdk1 control cells (Cdk1lox/lox;−) or Cdk1lox-cre cells (Cdk1lox/lox; Cre) 3 days after treatment with TAM or with vehicle (veh.) only (top) or, Plk1 control cells (Plk1+/lox; Cre) or Plk1lox-cre cells (Plk1lox/lox; Cre) 4 days after treatment with TAM or with veh only (bottom), relative to keratinocytes treated with veh. b Representative flow-cytometry histograms for the differentiation marker keratin K10 (K10+, positive cells according to negative isotype antibody control, not shown). Bar histograms show the percent of K10 positive cells as in a relative to keratinocytes treated with veh. c Representative flow-cytometry analyses of DNA content. Bar histograms: percent of 4C cells (G2/M + tetraploids), >4C cells (Polyploid) or in the Sub-G1 fraction of the cell cycle (as measured by PI), as in a and relative to keratinocytes treated with veh. **P < 0.01. Cdk1 control mice do not have CRE recombinase (−). Data are mean ± SD of duplicate or triplicate samples (n = 2 or n = 3) of at least two mice per group (N = 2) and three independent experiments. See also Supplementary Figs. 3, 4.