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. 2020 Feb 20;27(8):2451–2467. doi: 10.1038/s41418-020-0515-2

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© The Author(s), under exclusive licence to ADMC Associazione Differenziamento e Morte Cellulare 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Primary cells from human epidermis were treated with vehicle (veh.), blebbistatin (Blebb.) or cytochalasin (Cyto.) for 48 h. a Clonogenic capacity of cells drug-released and plated (3000 cells per well in triplicate samples) after treatment as indicated. b Representative flow-cytometry analyses for DNA content, morphology (light scattering) and expression of the differentiation marker involucrin (INV+, positive cells), as indicated. c Bar histograms: percent of 2C (G1, dyploid), 4C (G2/M + tetraploids) or >4C (polyploid) cells as measured by PI; percent of cells with high light scatter features (high SC); percent of INV+ cells; or percent of keratin K16 positive cells, as indicated. **P < 0.01. Data are mean ± SD of triplicate samples (n = 3) and three independent experiments. See also Supplementary Fig. 4.