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. 2020 Feb 20;27(8):2451–2467. doi: 10.1038/s41418-020-0515-2

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© The Author(s), under exclusive licence to ADMC Associazione Differenziamento e Morte Cellulare 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Apoptotic sub-G1 fraction as measured by PI, in control cells (CDC20+/+; Cre), upon vehicle only (48 h), Plk1 inhibitor BI2536 (48 h), lethal UV irradiation (48 h) or TAM for 3 days, as indicated; black bar for CDC20 KO; Cre cells upon TAM treatment for 3 days. Percent of cells 4C (G2/M + tetraploids) and >4C (polyploids) 48 h after BI2536 or UV irradiation, or 4 days after TAM. Fraction of cells positive for squamous differentiation markers K1 and K10 48 h after BI2536 or UV irradiation, or 3 days after TAM. c Frame from a representative time-lapse video by phase-contrast microscopy and DNA labelling by NucBlue, 31 h after addition of TAM to delete CDC20. Scale bar: 50 μm. b Flow-cytometry analyses for detection of apoptotic early marker Annexin V after BI2536 (48 h), UV irradiation (48 h) or TAM (4 days). Bar histogram displays quantification of Annexin positive cells. **P < 0.01, *P < 0.05, ns not significant. Data are mean ± SD of duplicate or triplicate samples (n = 2 or n = 3) of at least two mice per group (N = 2) and five independent experiments. See also Supplementary Fig. 8.