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. 2020 Mar 3;27(8):2517–2530. doi: 10.1038/s41418-020-0519-y

Fig. 1. Genome stability is impaired by depletion of essential autophagic proteins.

Fig. 1

a, e Immunoblot analysis using the indicated antibodies was performed after nuclear fractionation. The density of indicated nuclear proteins was normalized to lamin A/C (n = 4 for Ku70, Ku80 in 1A and n = 3 for cGAS in 1E). *p < 0.05, **p < 0.01 (Student’s t test). b Immunoblot analysis using the indicated antibodies was performed. The density of indicated proteins was normalized to tubulin (n = 3). *p < 0.05, **p < 0.01 (Student’s t test). c After treating the indicated cells with camptothecin (CPT), 1 μM for 2 h or not, immunoblot analysis using the indicated antibodies was performed. The density of indicated proteins was normalized to tubulin (n = 3). *p < 0.05, **p < 0.01 (Student’s t test). d After treating the indicated cells with camptothecin (CPT), 1 μM for 2 h or not and then performing nuclear fractionation, immunoblot analysis using the indicated antibodies was performed. f Neutral comet assay was performed, and cells were visualized using a fluorescence microscope. The data from n > 50 cells were analyzed and digitalized using OpenComet (n = 55 for MEF WT, and n = 52 for MEF ATG5KO). *p < 0.05, **p < 0.01 (Student’s t test).