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. 2020 Mar 3;27(8):2517–2530. doi: 10.1038/s41418-020-0519-y

Fig. 3. STAT1 induction positively regulates ISG15 expression after Atg5 or Atg7 depletion.

Fig. 3

a–e Immunoblot analysis using the indicated antibodies was performed. The density of indicated proteins was normalized to tubulin (n = 4 for a, b, and d, n = 3 for c and e). *p < 0.05, **p < 0.01 (Student’s t test). f Immunoblot analysis using the indicated antibodies was performed after nuclear fractionation. The density of indicated nuclear proteins was normalized to lamin A/C (n = 3 for STAT1, and n = 4 for phosphorylated STAT1). *p < 0.05, **p < 0.01 (Student’s t test). g After treating indicated cells with bafilomycin A1 (Baf.A1), 1 μM for 2 h or not, immunoblot analysis using the indicated antibodies was performed. h, j After transfection with the indicated siRNAs (50 pmol) for 48 h, immunoblot analysis using the indicated antibodies was performed. i After transfection with the indicated siRNAs (50 pmol) for 48 h, relative mRNA levels of the indicated gene in whole cell lysates were measured by real-time PCR. The mRNA levels were normalized to β-actin (n = 3). *p < 0.05, **p < 0.01 (Student’s t test). k After transient transfecting plasmids pCMV-SPORT6 and pCMV-SPORT6-mISG15 into cells, immunoblot analysis using the indicated antibodies was performed.