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. 2020 Mar 3;27(8):2517–2530. doi: 10.1038/s41418-020-0519-y

Fig. 6. Cytosolic dsDNA pathway regulates the expression of STAT1-ISG15.

Fig. 6

a After transfecting cells with or without G3-YSD (10 μg/ml) for 24 h, immunoblot analysis using the indicated antibodies was performed. b The density of indicated proteins in a was normalized to tubulin (n = 3). *p < 0.05, **p < 0.01 (Student’s t test). c After treating cells with or without 2′3′-cGAMP (5 μg/ml) for 24 h, immunoblot analysis using the indicated antibodies was performed. d The density of indicated proteins in c was normalized to tubulin (n = 3). *p < 0.05, **p < 0.01 (Student’s t test). e After transfecting cells with or without G3-YSD (10 μg/ml) for 24 h, immunoblot analysis using the indicated antibodies was performed. f After treating cells with or without RU.521 (2 μg/ml) for 24 h, immunoblot analysis using the indicated antibodies was performed. g The density of indicated proteins in f was normalized to tubulin (n = 3). *p < 0.05, **p < 0.01 (Student’s t test). h After transfecting cells with the indicated siRNAs (50 pmol) for 48 h, immunoblot analysis using the indicated antibodies was performed.