Fig. 3. KIAA1429 is essential for oocyte developmental competence and Kiaa1429Zp3cKO oocytes exhibit abnormal chromatin organization and RNA granules.
a GV oocytes were isolated from 3-week-old Kiaa1429Zp3cKO and control ovaries following superovulation in vivo and cultured for 14 h in vitro. A large number of Kiaa1429Zp3cKO oocytes were arrested at the GV stage, whereas the oocytes from control mice developed into MII oocytes. Scale bars: 100 μm. b Proportions of GVBD oocytes in Kiaa1429Zp3cKO and control GV oocytes cultured in vitro. Data are presented as the mean ± s.e.m. from three independent experiments (total number of oocytes analyzed: n = 83 for control; n = 84 for Kiaa1429Zp3cKO). ***P < 0.001, t test (two-tailed). c Proportions of oocytes with extruded first polar bodies (PBE) in Kiaa1429Zp3cKO and control GV oocytes cultured in vitro. Data are presented as the mean ± s.e.m. from three independent experiments (total number of oocytes analyzed: n = 83 for control; n = 84 for Kiaa1429Zp3cKO). ***P < 0.001, t test (two-tailed). d Kiaa1429Zp3cKO and control GV oocytes cultured with 5-ethynyl uridine (EU). The signals of nascent RNA are detected as red fluorescence, whereas the nuclei are blue (Hoechst). The oocyte morphology was observed by differential interference contrast (DIC) microscopy. Arrowheads indicate abnormal cytoplasmic granules. The images are representative of three independent experiments. Scale bars: 20 μm. e Percentage of GVBD oocytes and oocytes with extruded PBEs respectively in NSN, Int and SN oocytes cultured in vitro. Data are presented as the mean ± s.e.m. from three independent experiments (total number of oocytes analyzed: n = 70). *P < 0.05, ***P < 0.001, t test (two-tailed). f The proportion of the NSN, Int and SN nucleolus types in Kiaa1429Zp3cKO and control GV oocytes (total number of oocytes analyzed: n = 66 for control; n = 53 for Kiaa1429Zp3cKO). ***P < 0.001, chi-square test.