Fig. 2. Expression patterns of apoptosis proteins separate resistant from synergistically responding cell lines.

a Baseline expression of pro- and anti-apoptotic proteins of the TRAIL pathway. Circles summarise 684 quantifications, and circle sizes represent relative expression amounts of the proteins between cell lines. Protein amounts are provided in Supplemental Table 1. b Percentage of the variance of the original dataset explained by PCs. PCs with an eigenvalue >1 were retained for further analysis. Accumulated “variance explained” is plotted in black. c Weight coefficient table. Bars represent the contributions of the respective proteins to the different PCs. d Cell lines positioned in a multidimensional space according to their individual protein expression profiles. The PC space shown was defined by the first three PCs. Circle sizes decrease with distance from the observer to aid 3D visualisation. e Colour coding indicates responsiveness of cell lines to IZI1551/Birinapant (orange = low response; blue = synergistic response). Table insert indicates accuracy of spatial segmentation between low and synergistic responders.