Fig. 5. Responses to IZI1551/Birinapant can be predicted for cells isolated from melanoma metastases.

a Quantification of apoptosis regulatory proteins in cells derived from melanoma metastases. Red coloured circles represent pro-apoptotic and green circles anti-apoptotic proteins. Circles summarise 285 quantifications, and circle sizes represent mean protein quantities determined from at least n = 3 independent experiments. Protein amounts are shown in Supplemental table 1. b Positioning of melanoma cells from patient metastases in the PC space defined by 2D cultured cell lines. Empty circles indicate positions of patient cells. Circle colours reflect expected responsiveness (blue) or resistance (orange), based on the LDA segmented PC space. The circle size decreases with distance from the observer to aid 3D visualization. c Experimental validation of primary melanoma cell responsiveness to IZI1551/Birinapant treatment. Cells were treated as indicated for 24 h. Cell death was measured by flow cytometry (PI uptake). Heat maps show the mean of n = 3 independent experiments.