Figure 7.

Linkage between compounds and ABA in whole achene stages. (a) Kinetics of abscisic acid (ABA) net production (expressed in ng/achene) at each developmental stage in whole achene (WA) during milk thistle fruit maturation (calculated from Table 1). (b) Relative quantification using RT-qPCR of RNA expression of 2 genes implicated in ABA flavonolignans biosynthesis (ABA1 and LEC2). (c) Pearson correlation of extraction compounds in whole achene stages were performed by PAST software. (* p < 0.05, n = 3). (d) Identification of ABA-responsive cis-acting elements located in gene promoter gene sequences (from blue to yellow indicating low to high number of each cis-acting element). Values are the mean ± SD of 3 independent measurements. Characteristics of cis-acting elements are provided in Table S5. Different letters indicate significant differences at p < 0.05.