Matrine inhibits translocation of NF-κB into nucleus in A549, DU145, and MIA PaCa-2 cells. (A) p65 translocation into nucleus was observed by immunocytochemical analysis. A549, DU145, and MIA PaCa-2 cells (2 × 104 cells/well) were treated with matrine (50 µM) for 24 h in 37 °C, 5% CO2 incubator. P65 was observed in red, nuclear was observed in blue color. Cells were analyzed by Olympus FluoView FV1000 confocal microscope (Tokyo, Japan). (B) A proposed model of the matrine effect on CXCR4 activation. All experiments were performed independently at least three times and representative data are shown. ***
p < 0.001, **
p < 0.01 vs. non-treated (NT) cells.