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. 2020 May 22;13(2):209–215. doi: 10.3892/mco.2020.2050

Figure 2.

Figure 2

Treatment schedule for the MTT assay. After 24 h of cell seeding, cells were cultured in OKM-NZ medium containing 0 or 1% OKGS-NZ growth supplement, or 1% OKGS-NZ and 2 µg/ml 5-FU. Then, both 5-FU treated or untreated cells were cultured in OKM-NZ medium containing 0 or 1% OKGS-NZ growth supplement and different concentrations of Elental®. After 24 h, the ability of the cells to proliferate was evaluated using an MTT assay. 5-FU, 5-fluorouracil; OKM-NZ, Oral Keratinocyte Medium (OKM)-New Zealand Origin BPE medium; OKGS-NZ, OKM-NZ complete medium consisting of OKM basal medium supplemented with 1% oral keratinocyte growth supplement-New Zealand Origin BPE.