Fig. 4.

The p53 Pathway Remains Highly Acetylated when p300 Levels are Limited. a GSEA pathway analysis of significantly regulated genes (based on RNA-seq) in p300 KD mESC compared to Ctl mESC. The normalized enrichment score (NES) is indicated for each gene set. b Fold-change RPKM (RNA-seq) of p300 KD versus Ctl for genes belonging to the p53 pathway. Effect of p53 on gene expression is indicated. c UpSet representation of regions of H3K27ac enrichment and overlap in Ctl and p300 KD mESCs. d Boxplot showing H3K27ac enrichment at lost, common and gained peaks after p300 KD. p < 2.2 × 10^− 16 for all comparisons by Wilcoxon rank sum test. The bottom and top of the boxes correspond to the 25th and 75th percentiles, and the internal band is the 50th percentile (median). The plot whiskers correspond to 1.5× interquartile range and outliers are excluded. e Motif enrichment analysis of lost and gained regions of H3K27ac enrichment after p300 KD. Logo and p-values are indicated for each motif. f Average profile of p53 ChIP-seq in WT mESCs at lost, common and gained regions of H3K27ac enrichment after p300 KD