. 2020 Jul 20;16:251. doi: 10.1186/s12917-020-02454-9

Table 1.

Experimental conditions evaluated

Condition	Composition	M. bovis	LAB
1	CM _{(1460 μL)} + MB _{(40 μL)}	☑	☑*
2	CM _{(1460 μL)} + L1 _{(40 μL)}		☑
3	CM _{(1000 μL)} + L2 _{(500 μL)}		☑
4	CM _{(1420 μL)} + MB _{(40 μL)} + L1 _{(40 μL)}	☑	☑
5	CM _{(960 μL)} + MB _{(40 μL)} + L2 _{(500 μL)}	☑	☑
6	SP4 _{(1420 μL)} + MB _{(40 μL)} + L1 _{(40 μL)}	☑	☑
7	SP4 _{(960 μL)} + MB _{(40 μL)} + L2 _{(500 μL)}	☑	☑

CM: Cervical mucus. MB: M. bovis. SP4: Specific medium for Mycoplasma spp. isolation (conditions without CM). L1: Lactobacillus spp. at a concentration of 3.24 × 10⁶ UFC/mL. L2: Lactobacillus spp. at a concentration of 3.24 × 10⁸ UFC/mL. LAB: Lactic acid bacteria. ☑: Counts of M. bovis and lactic acid bacteria were made.* Lactic acid bacteria were isolated in the cervical mucus pool. The experimental conditions were prepared following a protocol previously described [25]