Table 3.
Condition | Composition | Hour (h) | pH1 | Log CFU/ml of M. bovis2 | Log CFU/ml of LAB3 |
---|---|---|---|---|---|
1 | CM + MB | 0 | 8.40a | 7.23a | 0a |
15 | 7.52a | 7.16a | 2.22a | ||
2 | CM + L1 | 0 | 8.40a | – | 1.81a |
15 | 6.64b | – | 7.40b | ||
3 | CM + L2 | 0 | 8.40a | – | 3.84a |
15 | 5.60b | – | 11.66b | ||
4 | CM + MB + L1 | 0 | 8.40a | 6.44a | 2.41a |
15 | 6.73b | 7.65b | 7.32a | ||
5 | CM+ MB + L2 | 0 | 8.40a | 7.56a | 3.20a |
15 | 5.60b | 0b | 9.75b | ||
6 | SP4 + MB + L1 | 0 | 7.60a | 7.38a | 1.93a |
15 | 7.19a | 8.72b | 4.34a | ||
7 | SP4 + MB + L2 | 0 | 7.60a | 7.45a | 3.31a |
15 | 7.21a | 8.19b | 5.44a |
a, b: Means with different superscripts between the times within conditions differ significantly (P < 0.05).1Standard error of the mean (SEM): 0.34; 2SEM: 0.11; 3 SEM: 1.86. CM: cervical mucus. MB: M. bovis. SP4: Specific medium for Mycoplasma spp. isolation (conditions without CM). L1: Lactobacillus spp. less concentrated (3.24 × 106 UFC/mL). L2: Lactobacillus spp. more concentrated (3.24 × 108 UFC/mL). LAB: Lactic acid bacteria