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. 2020 Jul 7;21(13):4808. doi: 10.3390/ijms21134808

Figure 3.

Figure 3

Marker expression comparison of iPSC-derived and peripheral blood mononuclear cells (PBMC)-derived CD14+ cells: (A) Heatmap comparing the differential expression of genes characteristic for macrophage polarization. Expression values are depicted as z scores. Red boxes indicate strongest expression among the different groups for each individual gene, and dark blue indicates lowest. Heatmap was generated using ClustVis [32]. iPSC-derived macrophage progenitors and macrophages were derived from SFC840-03-01 (STBCi026-B) and compared to commercial PBMC cells from a single donor. (B) Flow cytometry results of myeloid marker Programmed death-ligand 1 (PD-L1) and CD14 of cells derived from either PBMC or iPSC and polarized as described in detail above. Data are means ± SEM (three independent experiments, iPSC-derived macrophage progenitors and macrophages were derived from SFC840-03-01 (STBCi026-B) and compared to cells derived from PBMC from different donors). Statistical significance was determined by two-way ANOVA with Tukey‘s post hoc test. *** p < 0.001. (C) Refractive index images of macrophages derived from iPSC (SBNeo1) or PBMC and polarized either in M1, M0, or M2. Images were acquired using Nanolive 3D cell explorer. Scale bar represents 20 µm.