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. 2020 Jul 20;39:139. doi: 10.1186/s13046-020-01633-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — MK5-AS1 was upregulated in CRC tissues. a. A Heatmap of RNA-Seq analysis of differentially expressed lncRNAs in CRC and corresponding normal tissues generated from RNA sequencing data from the TCGA database. Red in the heatmap denotes upregulation, green denotes downregulation. b. Expression of MK5-AS1 in the TCGA CRC cohort. c. qPCR was utilized to analyze the MK5-AS1 expression in 172 pairs of CRC tissues and corresponding adjacent non-tumoral tissues. d. Expression of MK5-AS1 was detected by qPCR in the normal colorectal epithelium cell line (FHC) and CRC cell lines. e. Kaplan-Meier overall survival was analyzed according to MK5-AS1 expression in TCGA cohort. f. Kaplan-Meier overall survival and relapse-free survival curves according to MK5-AS1 expression in our cohort. *P < 0.05, **P < 0.01 and ***P < 0.001