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. 2020 Jul 20;39:139. doi: 10.1186/s13046-020-01633-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — MK5-AS1 regulated CRC invasion and migration in vitro and vivo. a. Transwell assays were used to determine the invasion and migration abilities of HCT116 and SW620 cells after MK5-AS1 overexpression and knockdown. Scale bar, 100 μm for A. b. Immunoblotting of EMT-related markers after transfection in CRC cells. c. Nude mice were injected with HCT116 cell after MK5-AS1 knockdown into tail vein. The number of metastatic nodules of lung was shown and counted. The black arrow marked metastatic nodules. d. Tumor progression was monitored using a small animal imaging system, HE-stained lung sections (Scale bar, 100 μm) and antibody vimentin of metastatic nodules were shown (× 400 magnification). The black arrow marked metastatic nodules. e. The Ensembl Genome browser (http://asia.ensembl.org/) showed that MK5 was the nearby gene of MK5-AS1. f. Immunoblotting was used to investigate the level of MK5 of HCT116 and SW620 cells after intervening MK5-AS1, respectively. The data represented the mean ± SD from three independent experiments. *P < 0.05, **P < 0.01 and ***P < 0.001