Fig. 7.

MK5-AS1/let-7f-1-3p/SNAI1 ceRNA network. a. Transwell assays were used to perform the invasion and migration abilities in HCT116 and SW620 cells after transfection. Scale bar, 100 μm for A. b. The potential binding sites among MK5-AS1, let-7f-1-3p and SNAI1. c. Upper panel, the potential binding sites between let-7f-1-3p and SNAI1. Lower panel, the luciferase reporter plasmids containing wild type (WT) or mutant (MUT) SNAI1 3′ UTR were cotransfected into HCT116 cells with let-7f-1-3p. d. MK5, c-Jun, p-c-Jun(S63) and SNAI1 expressions were detected in HCT116 and SW620 cells by immunoblotting after transfection with let-7f-1-3p inhibitor or let-7f-1-3p mimics. e. Left panel, the effects of si-MK5-AS1, let-7f-1-3p inhibitor and si-MK5-AS1 + let-7f-1-3p inhibitor on protein levels of SNAI1 in HCT116 and SW620 cells. Right panel, the effects of pcDNA3.1 MK5-AS1, let-7f-1-3p mimics, and pcDNA3.1 MK5-AS1 + let-7f-1-3p mimics on protein levels of SNAI1 in HCT116 and SW620 cells. Data were shown as mean ± SD for three independent experiments. *P < 0.05, **P < 0.01 and ***P < 0.001