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. 2020 Jul 20;18:100. doi: 10.1186/s12951-020-00656-9

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Assessment of LDEVs’ effect on gastric cancer cells. a Flow cytometry analysis of cell cycle phases of AGS, BGC-823 and SGC-7901 treated with LDEVs; b CCK-8 assay to evaluate the cell viability of AGS, BGC-823, and SGC-7901 cells treated with different concentration LDEVs; c Western blot analysis the expression of caspase 3 and cleaved caspase 3 proteins in gastric cancer cells; d Flow cytometry analysis the apoptosis of three gastric cancer cells induced by LDEVs; e Plate colony formation assay of AGS, BGC-823, and SGC-7901 cells with or without LDEVs treatment; f Fluorescence images of the live/dead staining 3D cultured AGS, BGC-823, and SGC-7901 cells (Scale bar = 100 μm). These experiments were performed three times