Table 4.

Physicochemical property analysis based on the amino acid sequences of the designed CTL and HTL MEVs.

Property		Cytotoxic T lymphocyte multiepitope vaccine	Helper T lymphocyte multiepitope vaccine
Length (amino acids)		704	810
Molecular weight (kilodaltons)		72.62	82.80
Theoretical protrusion index		9.70	8.64
Expected half-life (hours)
	Escherichia coli	10	10
	Yeast	30	30
	Mammalian cell	20	20
Aliphatic index		61.09	96.43
Grand average of hydropathicity		–0.090	0.501
Instability index		44.31	40.28