Figure 4.

OPT imaging of freshly excised whole mouse tibialis anterior (TA) muscle. (a) 3D OPT image of the TA muscle, where the highlighted regions had significant fiber disorganization. Cross-sectional images of (b) OCT intensity and (c) variation of fiber orientation in the same TA muscle. (d) Hematoxylin and eosin (H&E)-stained histology from the same sample. (e) The absolute 3D fiber orientation acquired in a TA muscle using dual-angle OPT. (f) Examples of individual bundles of muscle fibers extracted from inside the TA muscle. The images in (a) to (d) are adapted from Wang et al.⁴¹; the images in (e) and (f) are adapted from Wang et al.⁴³ (A color version of this figure is available in the online journal.)