IDE was significantly increased in eNOS+/− microvascular tissue. (a)
Microvascular tissue from 18-month-old eNOS+/− and wild type animals was
Western blotted using anti-ECE1, anti-IDE, anti-neprilysin, and anti-Actin (loading
control) antibodies. Representative image is shown. Densitometric analysis was
performed for (b) ECE1 (n = 12 animals), (c) IDE
(n = 7 animals; ***P < 0.0001), and (d)
neprilysin (n = 15 animals). All data are presented as mean ±SD
with individual data points shown.